An Overview of HPTLC with Chemometrics

Abstract

High-Performance Thin-Layer Chromatography (HPTLC) is a powerful method for the qualitative and quantitative assessment of complex mixtures in medicines, herbal products, and food analysis when combined with chemometric analysis. HPTLC enables efficient separation with high resolution, and chemometrics uses multivariate statistical methods like PCA and PLS to enhance data interpretation. This integration allows for the exact identification of markers and adulterants by improving accuracy, repeatability, and pattern recognition. Because the procedure is rapid, affordable, and environmentally benign, it is suitable for routine quality monitoring and authentication. While used together, HPTLC and chemometrics enhance analytical skills and ensure accuracy while evaluating multi-component samples in a range of scientific fields.