Journal of Pharmaceutical Quality Assurance and Quality Control

Indian Medicinal Plants Anti-Inflammatory Properties: An Extensive Analysis

2025-07-10T11:31:09+00:00

Many acute and chronic diseases are mostly caused by inflammation, a vital biological reaction. As worries about the negative effects of traditional anti-inflammatory medications, such as non-steroidal anti-inflammatory medicines (NSAIDs), have grown, attention has turned to looking at safer plant-based substitutes. Bioactive substances, including flavonoids, alkaloids, tannins, and terpenoids, which have strong anti-inflammatory effects, are abundant in Indian medicinal plants, which have their roots in traditional medical systems. The suppression of cyclooxygenase (COX), lipoxygenase (LOX), nitric oxide synthase (NOS), and pro-inflammatory cytokines are some of the methods by which these substances manipulate inflammation that are highlighted in this study. The significance of both in vitro and in vivo screening techniques for assessing these plants anti-inflammatory properties is also emphasized. In order to give readers a thorough grasp of the therapeutic potential of Indian medicinal herbs, this article will synthesize recent research and provide insightful information for future studies and the creation of safer and more potent anti-inflammatory drugs.

Basic Extraction and Fractionation Techniques for Experimental Using the HPLC Validation in the Preparation of Medicinal Plants

2025-07-17T09:16:35+00:00

Isolation and purification of particular bioactive components are frequently required when preparing medicinal plants for experimental usage. The fundamental extraction and fractionation technologies frequently used in this process are described in this paper, with a focus on the critical role that High-Performance Liquid Chromatography (HPLC) plays in validation and quality control. The goal of first extraction is to solubilise a wide variety of plant elements, usually by solvent-based techniques like maceration or Soxhlet extraction. Then, using further fractionation procedures such liquid-liquid extraction or solid-phase extraction, target chemicals are selectively enriched or complicated mixtures are separated according to their physicochemical characteristics. It is crucial that HPLC be incorporated into these preliminary procedures. By measuring the recovery of target analytes in the crude extract, HPLC is a potent analytical tool for confirming extraction effectiveness. Ensuring the presence and purity of the intended constituents in the final preparation through quality control and monitoring the success of fractionation by evaluating the enrichment of target compounds across various fractions. To ensure the dependability of the analytical data used to direct and assess the preparation process, method validation of the HPLC assay is crucial. This includes criteria such as linearity, accuracy, precision, and limits of detection and quantification. This integrated methodology offers a reliable method for producing well-characterized medicinal plant preparations appropriate for experimental studies by combining fundamental extraction and fractionation with exacting HPLC validation

Extraction, Investigation of Phytochemical Composition, and In Vitro Antioxidant Activity of Indigofera cordifolia

2025-08-02T10:27:55+00:00

Numerous diseases, such as cancer, diabetes mellitus, aging, and arthritis, are linked to free radicals. Antioxidant therapy has become increasingly important in the management of several illnesses. The main objective of the research is the extraction, phytochemical screening, and invitro antioxidant activity of Indigofera cordifolia. The standard methods employed methanol extracts, ethyl acetate, and chloroform for the initial phytochemical screening. Therefore, using a variety of conventional invitro models, a modern investigation sought to investigate the antioxidant properties of Indigofera cordifolia. The methanol extracts of Indigofera cordifolia were assessed in this endeavor for their ability to block radicals via the use of superoxide radical action, DPPH, lipid per oxidation assay, and the nitric oxide method. These extracts' phytochemical analysis revealed that the plant contains alkaloids, glycosides, terpenoids, steroids, and flavonoids. When compared to conventional antioxidants, the revealed results of the plant extract demonstrated a higher capability for radical scavenging activity. Similarly, a variety of characteristics demonstrated that in vitro extracts of Indigofera cordifolia had excellent antioxidant activity. According to recent research, the evidence provides useful information that can help select and conduct future research.

Quantification and Method Validation of Enalapril Maleate by Using Rp-Hplc

2025-09-26T04:39:15+00:00

A rapid, economical, and highly accurate RP-HPLC method was established for the determination of enalapril maleate in both bulk drug and pharmaceutical formulations. Analysis was carried out using a Waters system equipped with an INTERSIL C-18 ODS column (250 × 4.6 mm, 5 μm particle size). The mobile phase consisted of acetonitrile and water in an 80:20 ratio, delivered at a flow rate of 1 ml/min, with detection at 208 nm. The retention time was observed at 3.2 minutes. The method exhibited linearity in the concentration range of 20–120 μg/ml, with a correlation coefficient of 0.9994. Sensitivity was high, with a limit of detection (LOD) of 0.1 μg/ml and a limit of quantification (LOQ) of 0.3 μg/ml. Validation followed International Conference on Harmonization (ICH) guidelines, and the method proved effective for the quantification of marketed formulations.