Basic Extraction and Fractionation Techniques for Experimental Using the HPLC Validation in the Preparation of Medicinal Plants

Abstract

Isolation and purification of particular bioactive components are frequently required when preparing medicinal plants for experimental usage. The fundamental extraction and fractionation technologies frequently used in this process are described in this paper, with a focus on the critical role that High-Performance Liquid Chromatography (HPLC) plays in validation and quality control. The goal of first extraction is to solubilise a wide variety of plant elements, usually by solvent-based techniques like maceration or Soxhlet extraction. Then, using further fractionation procedures such liquid-liquid extraction or solid-phase extraction, target chemicals are selectively enriched or complicated mixtures are separated according to their physicochemical characteristics. It is crucial that HPLC be incorporated into these preliminary procedures. By measuring the recovery of target analytes in the crude extract, HPLC is a potent analytical tool for confirming extraction effectiveness. Ensuring the presence and purity of the intended constituents in the final preparation through quality control and monitoring the success of fractionation by evaluating the enrichment of target compounds across various fractions. To ensure the dependability of the analytical data used to direct and assess the preparation process, method validation of the HPLC assay is crucial. This includes criteria such as linearity, accuracy, precision, and limits of detection and quantification. This integrated methodology offers a reliable method for producing well-characterized medicinal plant preparations appropriate for experimental studies by combining fundamental extraction and fractionation with exacting HPLC validation