Synthesis and Structural Characterization of New Compound N- Benzamideceftriaxone and Assessment of Anti-Microbial Analysis

Abstract

Aim and Objective: We at this moment present the synthesis of N-Benzamideceftriaxone derived from the chemical precursors, Benzoic acid, and Ceftriaxone. The primary objective of this investigative study is to enhance the antibacterial efficacy of existing antibiotic agents and to establish a novel therapeutically active compound.

Methods: The synthesized chemical compound underwent comprehensive characterization through UV-visible spectroscopy, FTIR spectroscopy, PMR and Carbon NMR analysis. Subsequently, the antimicrobial properties of Ceftriaxone, Benzoic acid and also N-Benzamideceftriaxone were methodically assessed against a range of bacterial strains, including E. coli, Pseudomonas aeruginosa, Salmonella typhi, Staphylococcus aureus, K pneumonia and Proteus utilizing the disk-diffusion method. Notably new compound N-Benzamideceftriaxone exhibited stability, had a melting point exceeding 225ºC and yielded positive results in functional group tests. Furthermore, the synthesis of the new compound N-Benzamideceftriaxone from the precursors Benzoic acid also Ceftriaxone was corroborated using UV-visible spectroscopy, FTIR spectroscopy and NMR analysis.

Results: The N-Benzamideceftriaxone displayed varying antimicrobial effectiveness when tested against different bacterial strains. Notably, the most substantial inhibitory effect, with a zone of inhibition measuring 42 mm, was observed against Proteus. Following closely, Salmonella typhi and Escherichia coli exhibited zone sizes of 40 mm each, surpassing the individual antibacterial activities of Benzoic acid and ceftriaxone.

Conclusion: The growth of every examined bacterium was markedly lowered by the synthesis of the ligand N-Benzamideceftriaxone. N-Benzamideceftriaxone is a stable, affordable compound with remarkable antibacterial activity against pathogenic bacteria that is synthesized using benzoic acid and ceftriaxone. After a deeper study, this ligand may be utilized as a model molecule for developing novel antibiotics.