Ceftrizxone Estimation Using UV Spectroscopy: A Validated Method Developed

Abstract

Background and Aim: There is currently no procedure for estimating ceftriaxone via UV spectroscopy. Ceftriaxone comes in a variety of pharmacological dose formulations. To date, no UV-visible spectrophotometric method has been reported for the estimation of ceftriaxone in its various pharmaceutical dosage forms. Existing methods mainly involve HPLC, capillary electrophoresis, or microbiological assays, which can be time-consuming and equipment-intensive. We used methanol as a solvent and a λmax of 241 nm, which was chosen based on the maximum absorbance, to create a straightforward and affordable UV spectrophotometric technique.  The technique showed good reproducibility and was validated in accordance with ICH Q2(R1) requirements for linearity, accuracy, precision, LOD, and LOQ.  This approach closes a gap in the existing literature and provides a useful substitute for routine analysis, particularly in environments with limited resources. It is a straightforward, economical, repeatable, and trustworthy spectroscopic technique.

Method: For Ceftriaxone, distilled water was utilized as the solvent. After preparing a drug stock solution and determining its maximum absorbance (λmax), it was scanned between 200 and 400nm.

Results: Ceftriaxone demonstrated a strong association and complied with Beer’s law. In distilled water, it displayed absorption peaks at 240nm. Beer’s law regression study revealed good concentration ranges in distilled water between 2 and 4 µg per ml, with a concurrence coefficient of 0.999 and a line equation Y=0.039x-0.039. None of the common pharmaceutical excipients has an interface. Repeatability, bearish, and day-to-day studies were conducted to ascertain the method’s precision. Recovery experiments from pharmaceutical dosage forms validated the method’s accuracy. The analysis’s findings were confirmed by a recovery study and statistical analysis. According to the criteria of ICH Q2 (R2), the analytical method was verified for a number of parameters.

Conclusion: The method developed is straightforward, quick, and precise, making it suitable for regular quality control evaluations of ceftriaxone assays in pharmaceutical dosage forms.