Antiasthmatic Potential of Methanolic Extracts of Pistacia integerrima

Anjali Thakur, Ankush Dhiman, Anjali Sharma, Nutan Sharma — Tue, 22 Jul 2025 00:00:00 +0000

An infection happens when a germ affects the body and causes problems by reacting with the immune system. Diseases caused by infections can spread from person to person. These infections can last a short time (acute) or a long time (chronic). Asthma, a multifactorial respiratory condition, particularly prevalent among pediatric populations, has prompted interest in alternative treatments with fewer side effects. Numerous medicinal plants have been traditionally employed for asthma management, including Glycyrrhiza glabra, Adhatoda vasica, Albizzia lebbeck, Ephedra sinica, and others, each containing bioactive compounds such as glycyrrhizin, vasicine, ephedrine, and flavonoids. Despite their widespread use, the pharmacological mechanisms underlying their therapeutic effects remain insufficiently understood. This highlights the urgent need for rigorous scientific validation through preclinical and clinical studies to ensure efficacy and safety, and to integrate these herbal remedies into evidence-based modern medicine.

A comprehensive pharmacognostic and phytochemical evaluation of Pistacia integerrima leaf galls. Key morphological traits included green coloration, short petioles, and a slightly bitter taste. Microscopic analysis revealed features such as simple trichomes, vascular bundles, stomata, and calcium oxalate crystals. Phytochemical screening confirmed the presence of various bioactive compounds, including alkaloids, flavonoids, tannins, saponins, and sterols. Methanol extraction yielded the highest extractive value (12.8% w/w), and TLC fingerprinting showed multiple distinct spots. The findings establish a standard reference for the authentication and quality control of Pistacia integerrima. Additionally, the methanolic extract demonstrated significant bronchodilatory activity, supporting its traditional use in treating asthma.

Ceftrizxone Estimation Using UV Spectroscopy: A Validated Method Developed

Krishna Jee, Meghna Misra, Shivani, Preeti, Kapil Rana, Rishabh Gaur — Thu, 11 Sep 2025 00:00:00 +0000

Background and Aim: There is currently no procedure for estimating ceftriaxone via UV spectroscopy. Ceftriaxone comes in a variety of pharmacological dose formulations. To date, no UV-visible spectrophotometric method has been reported for the estimation of ceftriaxone in its various pharmaceutical dosage forms. Existing methods mainly involve HPLC, capillary electrophoresis, or microbiological assays, which can be time-consuming and equipment-intensive. We used methanol as a solvent and a λmax of 241 nm, which was chosen based on the maximum absorbance, to create a straightforward and affordable UV spectrophotometric technique. The technique showed good reproducibility and was validated in accordance with ICH Q2(R1) requirements for linearity, accuracy, precision, LOD, and LOQ. This approach closes a gap in the existing literature and provides a useful substitute for routine analysis, particularly in environments with limited resources. It is a straightforward, economical, repeatable, and trustworthy spectroscopic technique.

Method: For Ceftriaxone, distilled water was utilized as the solvent. After preparing a drug stock solution and determining its maximum absorbance (λmax), it was scanned between 200 and 400nm.

Results: Ceftriaxone demonstrated a strong association and complied with Beer’s law. In distilled water, it displayed absorption peaks at 240nm. Beer’s law regression study revealed good concentration ranges in distilled water between 2 and 4 µg per ml, with a concurrence coefficient of 0.999 and a line equation Y=0.039x-0.039. None of the common pharmaceutical excipients has an interface. Repeatability, bearish, and day-to-day studies were conducted to ascertain the method’s precision. Recovery experiments from pharmaceutical dosage forms validated the method’s accuracy. The analysis’s findings were confirmed by a recovery study and statistical analysis. According to the criteria of ICH Q2 (R2), the analytical method was verified for a number of parameters.

Conclusion: The method developed is straightforward, quick, and precise, making it suitable for regular quality control evaluations of ceftriaxone assays in pharmaceutical dosage forms.

Research & Review: Drugs and Drugs Development (e-ISSN: 2582-5720)

Antiasthmatic Potential of Methanolic Extracts of Pistacia integerrima

Ceftrizxone Estimation Using UV Spectroscopy: A Validated Method Developed