Pharmacological and Toxicological Assessment of Anti-Arthritic and Anti-Inflammatory Activity of Vitex trifolia

Abstract

Objective: In order to assess the anti-arthritic properties of Vitex trifolia, research was carried out through both in-vitro and in-vivo methodologies. Methods: The current investigations were conducted on Wistar rats that had been given 0.1 ml of FCA to cause Arthritis and 0.1 ml of carrageenan to induce paw edema. Consequently, VTE and VTA inhibition was measured as a percentage. Additionally, in-vitro techniques utilizing Egg’s albumin protein denaturation method and HRBC cell membrane stabilization technique were employed to discover anti-inflammatory and anti-arthritic characteristics. The ethanolic and aqueous extracts of Vitex trifolia were evaluated and compared to the standard medication indomethacin. Results: In our result it is found that VTE at concentration of 800μg/ml exhibited maximum 97.50% protection respectively and VTA at same concentration showed 83.76 % protection whereas Standard indomethacin exhibited 90.05% protection of HRBC in hypotonic solution respectively. In protein denaturation method, VTE showed 201% inhibition at concentration of 2000 μg/ml and VTA showed 129 % inhibition whereas standard indomethacin showed 176.85 % inhibition. In the in-vivo method, groups treated with VTA and VTE at doses of 100 mg/kg of b.w and 200 mg/kg of b.w, along with indomethacin at 10 mg/kg of b.w, a marked decline in paw volume was observed, along with a consistent increase in b.w relative to the +ve control group. Conclusions:  The stability of the HRBC membrane and suppression of protein denaturation were both concentration-dependent in current research. The findings imply that Vitex trifolia may protect rats from developing Arthritis and inflammation brought on by FCA and carrageenan